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KMID : 0380020110260060552
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Korean Journal of Biotechnology and Bioengineering
2011 Volume.26 No. 6 p.552 ~ p.556
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Isolation of a Marine-derived Flammeovirga sp. mbrc-1 Strain and Characterization of Its Agarase
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Jang Hye-Ji
Lee Dong-Geun
Lee Seung-Woo
Jeon Myong-Je
Chun Won-Ju
Kwon Kae-Kyoung
Lee Hee-Soon
Lee Sang-Hyeon
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Abstract
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A novel agar-degrading bacterium mbrc-1 was isolated from seashore of Kyungpo at Gangwon province and cultured in marine broth 2216 medium. Isolated bacterium mbrc-1 was named as Flammeovirga sp. mbrc-1 based on the 16S rDNA sequence. Its agarase showed maximum activity of 923 units/L at pH 7.0 and 45oC and sustained 90% remaining activity after exposed to 45oC for 2 hours. The enzyme hydrolyzed agarose to yield neoagarohexaose (18.5%), neoagarotetraose (38%) and neoagarobiose (43.5%), indicating that the enzyme is β-agarase. Thus, isolated bacterium and its β-agarase would be useful for the industrial production of neoagarotetraose and neoagarobiose.
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KEYWORD
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-agarase, agarose, Flammeovirga sp., neoagarobiose, neoagarotetraose
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